Current Genetics

, Volume 10, Issue 6, pp 435–441

Cloned mitochondrial DNA from the zygomycete Absidia glauca promotes autonomous replication in Saccharomyces cerevisiae

  • Anke Burmester
  • Johannes Wöstemeyer
Original Articles

DOI: 10.1007/BF00419870

Cite this article as:
Burmester, A. & Wöstemeyer, J. Curr Genet (1986) 10: 435. doi:10.1007/BF00419870

Summary

We have cloned fragments from mitochondrial and chromosomal DNA of the zygomycete Absidia glauca in Saccharomyces cerevisiae using the ARS selection vector YIp5. Though it has not been possible to select ARS elements from chromosomal DNA, we succeeded in isolating two clones of mitochondrial origin that support autonomous replication in bakers' yeast. DNA from these plasmids has been shown to hybridize with mitochondrial DNA from both mating types. Generation times of the transformed yeast strain in selective medium are around 20 h. In liquid minimal medium only 6% of the cells contain the plasmid; in complete medium a mitotic stability of 50% has been determined.

Key words

ARSMitochondrialDNAAbsidia glauca

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Anke Burmester
    • 1
  • Johannes Wöstemeyer
    • 1
  1. 1.Institut für Biochemie und Molekulare Biologie/BotanikTechnische UniversitätBerlin 10Germany