The localization of a lectin-like component on the Leishmania cell surface
- Cite this article as:
- Hernández, A.G., Rodríguez, N., Stojanovic, D. et al. Mol Biol Rep (1986) 11: 149. doi:10.1007/BF00419735
- 15 Downloads
The binding between marcrophage-like cells J774G8 and Leishmania braziliensis (NR) promastigotes was studied ‘in vitro’ by a radioisotopic assay under various conditions in the absence of serum. Different sugars, N-acetyl-D-glucosamine, D-glucose, D-mannose, D-galactose, and chitin, diminished the binding of the parasite, whereas other sugars, D-arabinose, D-fucose and D-xylose, did not affect the binding. The presence of a lectin-like ligand specific for N-acetyl-D-glucosamine has been detected on the cell surface of the Leishmania braziliensis (NR) by fluorescence microscopy.
These data suggest that the binding of the parasite to the host's cell is a ligand-receptor interaction which involves the participation of a lectin-like component on the parasite cell surface.