Current Genetics

, Volume 12, Issue 7, pp 503–509

Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number

  • Hans Koff
  • Cornelia Schmidt
  • Gerlinde Wiesenberger
  • Carlo Schmelzer
Original Articles

DOI: 10.1007/BF00419559

Cite this article as:
Koff, H., Schmidt, C., Wiesenberger, G. et al. Curr Genet (1987) 12: 503. doi:10.1007/BF00419559

Summary

A gene bank of a yeast wild type DNA in the high copy number vector YEp 13 was screened for recombinant plasmids which suppress the mitochondrial RNA splice defect exerted by mutant M1301, a −1 by deletion in the first intron of the mitochondrial COB gene (bIl). A total of 17 recombinant plasmids with similar suppressor activity were found. Restriction mapping and cross-hybridization of the inserts revealed that these 17 plasmids contain three different inserts, all lacking any extended sequence homology. Each of the inserts, when present in high copy number, has a similar suppressor activity: high in the presence of mutation M1301 in bll, a group II intron, and low but significant with the presence of few mutants in bI2 and bI3 of the COB gene, both of which are group I introns.

Key words

Suppression by high copy numberRNA splicingYeast mitochondria

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Hans Koff
    • 1
  • Cornelia Schmidt
    • 1
  • Gerlinde Wiesenberger
    • 1
  • Carlo Schmelzer
    • 1
  1. 1.Institut für Genetik und MikrobiologieUniversität MünchenMünchen 19Germany
  2. 2.Institut für Physiologische ChemieMünchen 2Germany
  3. 3.Max-Planck-Institut für BiochemieMünchen 2ViennaGermanyAustria
  4. 4.Institut für Genetik und MikrobiologieUniversität WienViennaAustria