Current Genetics

, Volume 12, Issue 7, pp 497–501

Amplification of the yeast nuclear gene MRS3 confers suppression of a mitochondrial RNA splice defect

  • Thomas Söllner
  • Cornelia Schmidt
  • Carlo Schmelzer
Original Articles

DOI: 10.1007/BF00419558

Cite this article as:
Söllner, T., Schmidt, C. & Schmelzer, C. Curr Genet (1987) 12: 497. doi:10.1007/BF00419558

Summary

The MRS3 gene cloned in the multicopy plasmid YEp13 suppresses the mitochondrial splice defect exerted by mutation M1301 in the group II intron bIl. In this article we report on the behavior of the MRS3 gene cloned in the integration vector pEMBLYi27 and in the CEN4-ARS vector YCp50. Transformation of mutant M1301 cells with these recombinant vectors produced transformants, the majority of which showed the original splice defect and contained the recombinant vectors in single or low copy; a minority, however, was splicing competent and showed exceptionally high copy numbers of the MRS3 gene. These latter transformants had either the pEMBLYi27/MRS3 sequence repeated at least 20 times in tandem at the chromosomal site of the MRS3 gene or they had the YCp50/MRS3 sequence established as a multicopy plasmid lacking the copy number control usually exerted by the CEN4 sequence in this plasmid.

Key words

Suppression by gene amplificationRNA splicingGroup II intronYeast mitochondria

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Thomas Söllner
    • 1
  • Cornelia Schmidt
    • 1
  • Carlo Schmelzer
    • 1
  1. 1.Institut für Genetik und MikrobiologieUniversität MünchenMünchen 19Germany
  2. 2.Institut für physiologische ChemieMünchen 2Germany
  3. 3.Max-Planck Institut für BiochemieMartinsriedGermany