Antonie van Leeuwenhoek

, Volume 54, Issue 2, pp 149–163

Isolation and characterisation of dog uropathogenic Escherichia coli strains and their fimbriae

Authors

  • E. Garcia
    • Institute of Infectious Diseases and Immunology, Dept. of Bacteriology, Faculty of Veterinary MedicineUniversity of Utrecht
  • H. E. N. Bergmans
    • Dept. of Molecular Cell Biology, Transitorium 3University of Utrecht
  • J. F. van den Bosch
    • Intervet International B.V.
  • I. Ørskov
    • Statens SeruminstitutInternational Escherichia coli Centre (WHO)
  • B. A. M. van der Zeijst
    • Institute of Infectious Diseases and Immunology, Dept. of Bacteriology, Faculty of Veterinary MedicineUniversity of Utrecht
  • W. Gaastra
    • Institute of Infectious Diseases and Immunology, Dept. of Bacteriology, Faculty of Veterinary MedicineUniversity of Utrecht
Article

DOI: 10.1007/BF00419202

Cite this article as:
Garcia, E., Bergmans, H.E.N., van den Bosch, J.F. et al. Antonie van Leeuwenhoek (1988) 54: 149. doi:10.1007/BF00419202

Abstract

A number of Escherichia coli strains have been isolated from dogs with urinary tract infections. These strains have been characterised with respect to their O, K, H, and fimbrial antigens, colicin production, antibiotic resistance, plasmid content and their ability to haemagglutinate erythrocytes from various species. Crossed immunoelectrophoresis of fimbrial extracts, as well as the reaction of partly purified fimbriae of a number of these strains with monoclonal antibodies revealed homology or a strong crossereaction with an F12 fimbrial subunit protein of human uropathogenic E. coli strains. Unlike human F12 fimbriae producing strains, the dog isolates did agglutinate dog erythrocytes in the presence of D-mannose but not human erythrocytes, indicating that the adhesin carried by these strains is different from the adhesin on fimbriae of human uropathogenic E. coli. Similar indications were obtained from experiments with latex beads coated with the receptor for P-fimbriae. These beads were agglutinated by Escherichia coli strains from human urinary tract infections, but not by the dog isolates described here. Preliminary adhesion experiments of human and dog Escherichia coli to human bladder epithelial and canine kidney epithelial cells also showed differences in adhesion depending on the origin of the strain tested.

Key words

Escherichia coliurinary tract infectiondogsfimbriae

Copyright information

© Kluwer Academic Publishers 1988