Current Genetics

, Volume 10, Issue 5, pp 339–342

The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c

  • J. Verdière
  • F. Creusot
  • L. Guarente
  • P. P. Slonimski
Original Articles

DOI: 10.1007/BF00418404

Cite this article as:
Verdière, J., Creusot, F., Guarente, L. et al. Curr Genet (1986) 10: 339. doi:10.1007/BF00418404

Summary

The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differentially regulated by the product of CYP1. The HAP1 gene codes for a product which has previously been proved to be necessary for the expression of the heme dependent CYC1-UAS1 cis regulatory sequence. In this article, we show by complementation and recombination that CYP1 and HAP1 are the same gene, moreover we identify hap1-1 as an iso2-cytochrome c underproducer mutation of the CYP1 gene.

Key words

Saccharomyces cerevisiaeCytochrome cRegulatory gene

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • J. Verdière
    • 1
  • F. Creusot
    • 1
  • L. Guarente
    • 2
  • P. P. Slonimski
    • 1
  1. 1.Centre de Génétique Moléculaire du C. N. R. S.Laboratoire pro pre associé a l'Université Pierre et Marie CurieGif-sur-YvetteFrance
  2. 2.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA