Molecular and General Genetics MGG

, Volume 249, Issue 6, pp 637–647

skippy, a retrotransposon from the fungal plant pathogen Fusarium oxysporum

  • Nuria Anava
  • M. Isabel G. Roncero
Original Paper

DOI: 10.1007/BF00418033

Cite this article as:
Anava, N. & Roncero, M.I.G. Molec. Gen. Genet. (1995) 249: 637. doi:10.1007/BF00418033

Abstract

A retrotransposon from the fungal plant pathogen Fusarium oxysporum f. sp. lycopersici has been isolated and characterized. The element, designated skippy (skp) is 7846 by in length, flanked by identical long terminal repeats (LTR) of 429 by showing structural features characteristic of retroviral and retrotransposon LTRs. Target-site duplications of 5 bp were found. Two long overlapping open reading frames (ORF) were identified. The first ORF, 2562 by in length, shows homology to retroviral gag genes. The second ORF, 3888 bp in length, has homology to the protease, reverse transciptase. RNase H and integrase domains of retroelement pol genes in that order. Sequence comparisons and the order of the predicted proteins from skippy indicate that the element is closely related to the gypsy family of LTR-retrotransposons. The element is present in similar copy numbers in the two races investigated, although RFLP analysis showed differences in banding patterns. The number of LTR sequences present in the genome is higher than the number of copies of complete elements, indicating excision by homologous recombination between LTR sequences.

Key words

RetroelementsFusarium oxysporumReverse transcriptase

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Nuria Anava
    • 1
  • M. Isabel G. Roncero
    • 1
  1. 1.Departamento de Genética, Facultad de CienciasUniversidad de CórdobaCórdobaSpain