Archives of Microbiology

, Volume 137, Issue 3, pp 188–193

Selective inhibition of transition from sexual agglutination to zygote formation by ethyl N-phenylcarbamate in Saccharomyces cerevisiae

  • Satoshi Hasegawa
  • Naohiko Yanagishima
Original Papers

DOI: 10.1007/BF00414541

Cite this article as:
Hasegawa, S. & Yanagishima, N. Arch. Microbiol. (1984) 137: 188. doi:10.1007/BF00414541
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Abstract

Effects of ethyl N-phenylcarbamate (EPC) on the mating reaction of Saccharomyces cerevisiae were studied, with special attention on the effect on the α pheromone action. EPC inhibited zygote formation at a concentration which promoted induction of sexual agglutinability. EPC enhanced agglutinability induction by α pheromone, but inhibited α-pheromone-induced formation of large pearshaped cells in a mating type. The enhancement of agglutinability induction was accompanied with increased production of a agglutination substance and inhibition of α pheromone inactivation. EPC arrested the cell cycle of a cells probably in the step controlled by CDC19, CDC35, cAMP etc., just before the step controlled by CDC28, α pheromone etc.

Key words

Agglutination substanceα PheromoneCell cycleEthyl N-phenylcarbamateMating reactionMicrotubulesSaccharomyces cerevisiae

Abbreviations

EPC

Ethyl N-phenylcarbamate

PBS

0.01 M phosphate buffer solution, pH 5.5

SPB

spindle pole body

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Satoshi Hasegawa
    • 1
  • Naohiko Yanagishima
    • 1
  1. 1.Biological Institute, Faculty of ScienceNagoya UniversityNagoyaJapan