Archives of Microbiology

, Volume 151, Issue 2, pp 171–176

Activation of aromatic acids and aerobic 2-aminobenzoate metabolism in a denitrifying Pseudomonas strain

  • Klaus Ziegler
  • Reiner Buder
  • Jochen Winter
  • Georg Fuchs
Original Papers

DOI: 10.1007/BF00414434

Cite this article as:
Ziegler, K., Buder, R., Winter, J. et al. Arch. Microbiol. (1989) 151: 171. doi:10.1007/BF00414434

Abstract

The initial reactions possibly involved in the acrobic and anaerobic metabolism of aromatic acids by a denitrifying Pseudomonas strain were studied. Several acyl CoA synthetases were found supporting the view that activation of several aromatic acids preceeds degradation. A benzoyl CoA synthetase activity (AMP forming) (apparent Km values of the enzyme from nitrate grown cells: 0.01 mM benzoate, 0.2 mM ATP, 0.2 mM coenzyme A) was present in aerobically grown and anaerobically, nitrate grown cells when benzoate or other aromatic acids were present. In addition to benzoate and fluorobenzoates, also 2-amino-benzoate was activated, albeit with unfavorable Km (0.5 mM 2-aminobenzoate). A 2-aminobenzoyl CoA synthetase (AMP forming) was induced both aerobically and anaerobically with 2-aminobenzoate as growth substrate which had a similar substrate spectrum but a low Km for 2-aminobenzoate (<0.02 mM). Anaerobic growth on 4-hydroxybenzoate induced a 4-hydroxybenzoyl CoA synthetase, and cyclohexanecarboxylate induced another synthetase. In contrast, 3-hydroxybenzoate and phenyl-acetate grown anaerobic cells appeared not to activate the respective substrates at sufficient rates. Contrary to an earlier report extracts from aerobic and anaerobic 2-aminobenzoate grown cells catalysed a 2-aminobenzoyl CoA-dependent NADH oxidation. This activity was 10–20 times higher in aerobic cells and appeared to be induced by 2-aminobenzoate and oxygen. In vitro, 2-aminobenzoyl CoA reduction was dependent on 2-aminobenzoyl CoA NAD(P)H, and oxygen. A novel mechanism of aerobic 2-aminobenzoate degradation is suggested, which proceeds via 2-aminobenzoyl CoA.

Key words

Aromatic compoundsBenzoic acid2-AminobenzoateAnthranilic acidAcyl CoA SynthetasesMonooxygenase2-Aminobenzoyl CoA reductase

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Klaus Ziegler
    • 1
  • Reiner Buder
    • 1
  • Jochen Winter
    • 1
  • Georg Fuchs
    • 1
  1. 1.Abteilung Angewandte MikrobiologieUniversität UlmUlmFederal Republic of Germany