Archives of Microbiology

, Volume 149, Issue 4, pp 372–376

Comparative analysis of the lipopolysaccharides of a rough and a smooth strain of Pseudomonas syringae pv. phaseolicola

  • M. Gross
  • H. Mayer
  • C. Widemann
  • K. Rudolph
Original Papers

DOI: 10.1007/BF00411658

Cite this article as:
Gross, M., Mayer, H., Widemann, C. et al. Arch Microbiol (1988) 149: 372. doi:10.1007/BF00411658


The lipopolysaccharides (LPS) of a rough (R) and a smooth (S) strain of Pseudomonas syringae pv. phaseolicola were analysed. The S-LPS revealed markedly more rhamnose and fucose, but less glucose, than the R-LPS. The presence of 3-O-methyl-rhamnose (acofriose) in the S-LPS was confirmed by cochromatography with authentic acofriose. SDS polyacrylamide gel electrophoresis of the S-LPS demonstrated a cluster of regularly spaced high molecular weight fractions, which was almost lacking in the R-LPS. The main fatty acids of the lipid A of both LPS species were 3-OH-10:0,3-OH-12:0,2-OH-12:0, and 12:0. Two N-linked diesters were demonstrated: 3-O(12:0)-12:0 and 3-O(2-OH-12:0)-12:0. S-LPS was subjected to mild hydrolysis and the “degraded polysaccharide” separated into three fractions by gel permeation chromatography on a Fractogel TSK HW-50 column. Fraction I, representing nearly only the O-specific side chain, consisted of rhamnose and fucose in a molar ratio of 4:1, with 4% of the rhamnose being 3-O-methylated (acofriose). Fraction II, representing mostly core material, was composed of glucose, rhamnose, heptose, glucosamine, galactosamine, alanine, and a still unidentified amino compound, in an approximate molar ratio of 3:1:1:1:1:1:1, and KDO. Fraction III consisted of released monomers and salts. The LPS was highly phosphorylated (3.28% phosphorus in the “core fraction”). The thus characterized composition of the LPS O-chain seems to be unique for the pathovar phaseolicola of P. syringae, although many similarities exist to other pathovars as well as to other bacterial species.

Key words

Lipopolysaccharides Pseudomonas syringae pv. phaseolicola R mutant Acofriose Halo blight of bush bean 





combined gas liquid chromatography-mass spectrometry


high voltage electrophoresis


2-keto-3-deoxyoctonic acid


polyacrylamide gel electrophoresis


sodium dodecylsulfate

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • M. Gross
    • 1
  • H. Mayer
    • 2
  • C. Widemann
    • 2
  • K. Rudolph
    • 1
  1. 1.Institut für Pflanzenpathologie und PflanzenschutzGöttingenFederal Republic of Germany
  2. 2.Max Planck-Institut für ImmunbiologieFreiburgFederal Republic of Germany

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