Original Articles

Current Genetics

, Volume 10, Issue 9, pp 657-664

First online:

Identification and characterization of four new GCD genes in Saccharomyces cerevisiae

  • Peter NiederbergerAffiliated withMikrobiologisches Institut, Eidgenössische Technische Hochschule
  • , Markus AebiAffiliated withMikrobiologisches Institut, Eidgenössische Technische Hochschule
  • , Ralf HütterAffiliated withMikrobiologisches Institut, Eidgenössische Technische Hochschule

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Summary

Mutant strains, resistant against the amino acid analogues 5-methyltryptophan, 5-fluorotryptophan and canavanine were isolated, starting with a trp2 leaky auxotrophic strain. Of 10 such strains, only four turned out to be of the “general control derepressed” (gcd) mutant type. Three other isolates were shown to be defective in the general amino acid permease system, while the remaining three strains displayed low spore viability and were not further investigated. Complementation tests amongst the four new gcd-mutant strains, including strain RH558 gcd2-1 isolated earlier, yielded five complementation groups: GCD2, GCD3, GCD4, GCD5, and GCD6. All mutant strains showed a dual phenotype, which was not separable by wild type backcrosses: “constitutive derepression” and “slow growth”. Epistatis of all gcd mutations over gcn1-1, gcn2-1 and gcn3-1 was found with respect to both phenotypes, except for gcd5-1, which was lethal in these combinations. On the other hand gcn4-101 was found to be epistatic over all gcd mutations, but only with respect to the “constitutive derepression” phenotype, and not to “slow growth”; again the combination with gcd5-1 was lethal. Mutation gcd2-1 was mapped on chromosome VII, 50 cM from leu1 and 22 cM from ade6. A new model is discussed, in which GCD-genes are involved in the amino acid uptake into the vacuoles.

Key words

Saccharomyces cerevisiae Amino acid biosynthesis General control GCD-genes GCN-genes