Archives of Microbiology

, Volume 150, Issue 1, pp 78–84

Degradation of chlorosubstituted aromatic compounds by Pseudomonas sp. strain B13: fate of 3,5-dichlorocatechol

  • Uwe Schwein
  • Eberhard Schmidt
  • Hans-Joachim Knackmuss
  • Walter Reineke
Original Papers

DOI: 10.1007/BF00409721

Cite this article as:
Schwein, U., Schmidt, E., Knackmuss, H. et al. Arch. Microbiol. (1988) 150: 78. doi:10.1007/BF00409721

Abstract

The degradation of 3,5-dichlorocatechol by enzymes of 3-chlorobenzoate-grown cells of Pseudomonas sp. strain B13 was studied. The following compounds were formed from 3,5-dichlorocatechol: trans-2-chloro-4-carboxymethylenebut-2-en-4-olide, cis-2-chloro-4-carboxymethylenebut-2-en-4-olide, and chloroacetylacrylate as the decarboxylation product of 2-chloromaleylacetate. They were identified by chromatographic and spectroscopic methods (UV, MS, PMR). An enzyme activity converting trans-2-chloro-4-carboxymethylenebut-2-en-4-olide into the cis-isomer was observed.

Key words

3,5-Dichlorocatechol degradationPseudomonas sp. strain B13trans-2-chloro-4-carboxymethylenebut-2-en-4-olide isomerase

Abbreviations

3CB

3-chlorobenzoate

4CB

4-chlorobenzoate

3,5DCB

3,5-dichlorobenzoate

2,4D

2,4-dichlorophenoxyacetate

NOE

Nuclear-Overhauser-Effect

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Uwe Schwein
    • 1
  • Eberhard Schmidt
    • 1
  • Hans-Joachim Knackmuss
    • 1
  • Walter Reineke
    • 1
  1. 1.Gesamthochschule WuppertalChemische Mikrobiologie der Bergischen UniversitätWuppertal 1Federal Republic of Germany
  2. 2.E. MerckDarmstadt 1Federal Republic of Germany
  3. 3.Institut für Mikrobiologie der Universität StuttgartStuttgart 1Federal Republic of Germany