Archives of Microbiology

, Volume 116, Issue 1, pp 69–75

Turnover of lipids in Mycobacterium smegmatis CDC 46 and Mycobacterium phlei ATCC 354

  • K. R. Dhariwal
  • A. Chander
  • T. A. Venkitasubramanian
Article

DOI: 10.1007/BF00408735

Cite this article as:
Dhariwal, K.R., Chander, A. & Venkitasubramanian, T.A. Arch. Microbiol. (1978) 116: 69. doi:10.1007/BF00408735

Abstract

The rates of breakdown and renewal of individual lipids in cultures of Mycobacterium smegmatis CDC 46 and Mycobacterium phlei ATCC 354 were investigated by means of a pulse labelling technique using palmitate-1-14C. The results indicated that in growing cultures of both strains phospholipids were broken down, and cardiolipin had a very rapid turnover. In chase experiments, almost 45% and 40% of the radioactivity of this component were lost respectively from M. smegmatis and M. phlei during one generation time of the cell. The other two major components, phosphatidyl ethanolamine and phosphatidylinositol mannosides showed relatively low turnover. The loss of radioactivity from phosphatidylinositol mannosides was greater in M. phlei than in M. smegmatis but the loss of radioactivity from phosphatidyl ethanolamine was higher in M. smegmatis. The pattern of loss of radioactivity from lipids was almost the same in both strains, the difference being only in the extent of loss. The differences in the cellular localization of the phospholipids indicate their different roles within the cell. Results obtained with the glyceride fraction indicated a very rapid turnover of triglycerides in both strains.

Key words

TurnoverCardiolipinPhosphatidyl ethanolaminePhosphatidylinositol mannosidesGlyceridesMycobacterium smegmatisMycobacterium phlei

Abbreviations

CL

Cardiolipin

PE

Phosphatidyl ethanolamine

PIMx

phosphatidylinositol mannosides

PIM2A

phosphatidylinositol dimannoside tetra acylated

PIM2B

phosphatidylinositol dimannoside tri acylated

PIM5

phosphatidylinositol pentamannoside tetra acylated

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • K. R. Dhariwal
    • 1
  • A. Chander
    • 1
  • T. A. Venkitasubramanian
    • 1
  1. 1.Department of Biochemistry, Vallabhbhai Patel Chest InstituteUniversity of DelhiDelhiIndia