Archives of Microbiology

, Volume 128, Issue 2, pp 253–255

The distribution of the NADPH regenerating mannitol cycle among fungal species

  • Karl Hult
  • Andres Veide
  • Sten Gatenbeck
Article

DOI: 10.1007/BF00406168

Cite this article as:
Hult, K., Veide, A. & Gatenbeck, S. Arch. Microbiol. (1980) 128: 253. doi:10.1007/BF00406168

Abstract

The mannitol cycle is an important NADPH regenerating system in Alternaria alternata. The cycle is built up of the following enzymes: mannitol 1-phosphate dehydrogenase, mannitol 1-phosphatase, mannitol dehydrogenase and hexokinase. The net reaction of one cycle turn is: NADH+NADP++ATP → NAD++NADPH+ADP+Pi. The enzymes needed for an operating cycle were found in Aspergillus, Botrytis, Penicillium, Pyricularia, Trichothecium, Cladosporium and Thermomyces all genera belonging to Fungi Imperfecti. The only genus of this class lacking the cycle was Candida. No genera from the classes Basidiomycetes and Phycomycetes showed any mannitol 1-phosphate dehydrogenase or mannitol 1-phosphatase activities. The genera investigated, belonging to Ascomycetes, Gibberella, Ceratocystis and Neurospora all lacked mannitol 1-phosphate dehydrogenase. It was concluded that the mannitol cycle is an important and widespread pathway for NADH oxidation and NADP+ reduction in the organisms belonging to the class Fungi Imperfecti.

Key words

Mannitol Mannitol cycle NADPH regeneration Fungi 

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Karl Hult
    • 1
  • Andres Veide
    • 1
  • Sten Gatenbeck
    • 1
  1. 1.Department of Biochemistry and BiotechnologyThe Royal Institute of TechnologyStockholmSweden