Archives of Microbiology

, Volume 128, Issue 2, pp 239–241

ATP-linked citrate lyase activity in the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum

  • R. N. Ivanovsky
  • N. V. Sintsov
  • E. N. Kondratieva
Article

DOI: 10.1007/BF00406165

Cite this article as:
Ivanovsky, R.N., Sintsov, N.V. & Kondratieva, E.N. Arch. Microbiol. (1980) 128: 239. doi:10.1007/BF00406165

Abstract

Cell-free extracts of the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum strains 1C and L have been shown to cleave citrate with the formation of oxaloacetate and acetyl-CoA. This capacity was found in autotrophically grown cells as well as in the cells grown on media with acetate or L-glutamate. Citrate lyase activity in cell-free extracts is only measurable in the presence of citrate, adenosine-5′-triphosphate, coenzyme A and Mg2+ or Mn2+. It is concluded on the basis of the obtained data that C. limicola f. thiosulfatophilum contains adenosine-5′-triphosphate-linked citrate lyase (E.C.4.1.3.8). In contrast to green bacteria in the purple bacteria Ectothiorhodospira shaposhnikovii, Rhodospirillum rubrum and Thiocapsa roseopersicina citrate lyase activity was not found.

Key words

Chlorobium limicola forma thiosulfatophilumATP-linked citrate lyase

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • R. N. Ivanovsky
    • 1
  • N. V. Sintsov
    • 1
  • E. N. Kondratieva
    • 1
  1. 1.Department of MicrobiologyMoscow State UniversityMoscowU.S.S.R.