ATP-linked citrate lyase activity in the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum
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- Ivanovsky, R.N., Sintsov, N.V. & Kondratieva, E.N. Arch. Microbiol. (1980) 128: 239. doi:10.1007/BF00406165
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Cell-free extracts of the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum strains 1C and L have been shown to cleave citrate with the formation of oxaloacetate and acetyl-CoA. This capacity was found in autotrophically grown cells as well as in the cells grown on media with acetate or L-glutamate. Citrate lyase activity in cell-free extracts is only measurable in the presence of citrate, adenosine-5′-triphosphate, coenzyme A and Mg2+ or Mn2+. It is concluded on the basis of the obtained data that C. limicola f. thiosulfatophilum contains adenosine-5′-triphosphate-linked citrate lyase (E.C.18.104.22.168). In contrast to green bacteria in the purple bacteria Ectothiorhodospira shaposhnikovii, Rhodospirillum rubrum and Thiocapsa roseopersicina citrate lyase activity was not found.