Current Genetics

, Volume 12, Issue 5, pp 305–310

Phenotypic suppression and nuclear accommodation of the mitoxi1-V25 mutation in isolated yeast mitochondria

  • W. Zagórski
  • M. Boguta
  • M. Mieszczak
  • M. Claisse
  • B. Guiard
  • A. Spyridakis
  • P. P. Slonimski
Original Articles

DOI: 10.1007/BF00405752

Cite this article as:
Zagórski, W., Boguta, M., Mieszczak, M. et al. Curr Genet (1987) 12: 305. doi:10.1007/BF00405752

Summary

Phenotypic suppression by the antibiotic, paromomycin, of the mitochondrial oxi1-V25 mutation, a mutation which arrests by premature ochre codon the synthesis of the cox 11 subunit, was studied in isolated yeast mitochondria competent in translation. This antibiotic is known to suppress the mutation in vivo (Dujardin et al. 1984) and allowed in vitro, at concentrations of 20–1100 Mg per ml. the synthesis of the cox II subunit. This strongly suggests that phenotypic suppression of mit mutations is due to the direct action of paromomycin on mitochondrial ribosomes. The effect of paromomycin bears a resemblance to the function of the omnipotent nuclear suppressor mutation R705. The nuclear suppression was expressed in isolated mitochondria; suppressor mutation influenced the structure of the mitoribosome. Therefore, it appears that mitoribosomes are indeed the common target in the phenotypical and genetic nuclear suppression of the oxi1-V25 mutation.

Key words

Yeast Mitochondria Translation Informational Suppression 

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • W. Zagórski
    • 1
  • M. Boguta
    • 1
  • M. Mieszczak
    • 1
  • M. Claisse
    • 2
  • B. Guiard
    • 2
  • A. Spyridakis
    • 2
  • P. P. Slonimski
    • 2
  1. 1.Institute of Biochemistry and BiophysicsPolish Academy of SciencesWarsawPoland
  2. 2.Centre du Génétique Moléculaire du CNRSLaboratoire propre associé à l'Université Pierre et Marie CurieGif-sur-YvetteFrance

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