Polymorphism and complexity of HLA-DR: evidence for intra-HLA-DR region crossing-over events
- Cite this article as:
- Bontrop, R.E., Tilanus, M.G.J., Mikulski, M.M.A. et al. Immunogenetics (1988) 27: 40. doi:10.1007/BF00404442
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HLA-DR molecules were isolated from HLA-DR3, −5, and −w6 positive homozygous B-cell lines by immunoprecipitation with monoclonal antibodies and analyzed by gel electrophoretic techniques. DNA isolated from the same cell lines was digested with the restriction enzyme Taq I and hybridized with a DR beta full-length cDNA probe. We demonstrated that certain DRβI alleles are found in combination with different DRβIII alleles as defined by Southern blotting, protein chemistry, a functional assay using purified protein derivative-specific T-cell lines, and, in one case, also alloreactive T-cell reagents. Our results indicate that within the family of HLA-DRw52-associated haplotypes DR beta chain genes may have been transferred from one haplotype to another. The implications of these findings are discussed.