Immunogenetics

, Volume 27, Issue 1, pp 40–45

Polymorphism and complexity of HLA-DR: evidence for intra-HLA-DR region crossing-over events

  • Ronald E. Bontrop
  • Marcel G. J. Tilanus
  • Marlies M. A. Mikulski
  • Dienne G. Elferink
  • Annemarie Termijtelen
  • Rene R. P. de Vries
  • Jon J. van Rood
  • Marius J. Giphart
Article

DOI: 10.1007/BF00404442

Cite this article as:
Bontrop, R.E., Tilanus, M.G.J., Mikulski, M.M.A. et al. Immunogenetics (1988) 27: 40. doi:10.1007/BF00404442

Abstract

HLA-DR molecules were isolated from HLA-DR3, −5, and −w6 positive homozygous B-cell lines by immunoprecipitation with monoclonal antibodies and analyzed by gel electrophoretic techniques. DNA isolated from the same cell lines was digested with the restriction enzyme Taq I and hybridized with a DR beta full-length cDNA probe. We demonstrated that certain DRβI alleles are found in combination with different DRβIII alleles as defined by Southern blotting, protein chemistry, a functional assay using purified protein derivative-specific T-cell lines, and, in one case, also alloreactive T-cell reagents. Our results indicate that within the family of HLA-DRw52-associated haplotypes DR beta chain genes may have been transferred from one haplotype to another. The implications of these findings are discussed.

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Ronald E. Bontrop
    • 1
  • Marcel G. J. Tilanus
    • 1
  • Marlies M. A. Mikulski
    • 1
  • Dienne G. Elferink
    • 1
  • Annemarie Termijtelen
    • 1
  • Rene R. P. de Vries
    • 1
  • Jon J. van Rood
    • 1
  • Marius J. Giphart
    • 1
  1. 1.Department of Immunohaematology and Blood BankUniversity HospitalAA LeidenThe Netherlands