Journal of Biomolecular NMR

, Volume 4, Issue 5, pp 631–644

Determination of a complete set of coupling constants in 13C-labeled oligonucleotides

  • H. Schwalbe
  • J. P. Marino
  • G. C. King
  • R. Wechselberger
  • W. Bermel
  • C. Griesinger
Research Paper

DOI: 10.1007/BF00404274

Cite this article as:
Schwalbe, H., Marino, J.P., King, G.C. et al. J Biomol NMR (1994) 4: 631. doi:10.1007/BF00404274

Summary

Three experiments are introduced to determine a complete set of coupling constants in RNA oligomers. In the HCCH-E.COSY experiment, the vicinal proton-proton coupling constants can be measured with high accuracy. In the P-FIDS-CT-HSQC experiment, vicinal proton-phosphorus and carbon-phosphorus couplings are measured that depend on the phosphodiester backbone torsion angles β and ε. In the refocussed HMBC experiment, vicinal carbon-proton couplings are measured that depend on the glycosidic torsion angle χ.

Keywords

J coupling constantsIsotopic labelingRNAOligonucleotide structurev0-v4 angleβ,ε-angleHCCH-E.COSYP-FIDS-HSQC

Copyright information

© ESCOM Science Publishers B.V. 1994

Authors and Affiliations

  • H. Schwalbe
    • 1
  • J. P. Marino
    • 2
  • G. C. King
    • 3
    • 4
  • R. Wechselberger
    • 1
  • W. Bermel
    • 5
  • C. Griesinger
    • 1
  1. 1.Institut für Organische ChemieUniversität FrankfurtFrankfurtGermany
  2. 2.Department of ChemistryYale UniversityNew HavenUSA
  3. 3.Department of Biochemistry and Cell BiologyRice UniversityHoustonUSA
  4. 4.School of Biochemistry and Molecular GeneticsUniversity of New South WalesKensingtonAustralia
  5. 5.Bruker Analytische Messtechnik GmbHRheinstettenGermany