Human Genetics

, Volume 73, Issue 3, pp 225–229

Direct regional assignment of the gene for vitamin D binding protein (Gc-globulin) to human chromosome 4q11-q13 and identification of an associated DNA polymorphism

  • Nancy E. Cooke
  • H. F. Willard
  • E. V. David
  • Donna L. George
Original Investigations

DOI: 10.1007/BF00401232

Cite this article as:
Cooke, N.E., Willard, H.F., David, E.V. et al. Hum Genet (1986) 73: 225. doi:10.1007/BF00401232

Summary

Using a characterized human vitamin D binding protein (DBP) cDNA probe and a panel of rodent X human somatic cell hybrids, we established the chromosomal location of the structural gene for DBP on human chromosome 4. In situ hybridization of 3H-labeled DBP cDNA to human metaphase chromosomes confirmed this assignment and allowed regional localization to bands 4q11–4q13. A restriction fragment length polymorphism associated with the DBP gene that should prove useful in future linkage studies was identified with the enzyme BamHI.

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Nancy E. Cooke
    • 1
    • 2
  • H. F. Willard
    • 4
  • E. V. David
    • 1
    • 2
  • Donna L. George
    • 2
    • 3
  1. 1.Department of MedicineUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Department of Human GeneticsUniversity of PennsylvaniaPhiladelphiaUSA
  3. 3.Howard Hughes Medical InstituteUniversity of PennsylvaniaPhiladelphiaUSA
  4. 4.Department of Medical GeneticsUniversity of TorontoOntarioCanada
  5. 5.555 Johnson PavilionUniversity of PennsylvaniaPhiladelphiaUSA