Original Investigations

Human Genetics

, Volume 73, Issue 3, pp 225-229

First online:

Direct regional assignment of the gene for vitamin D binding protein (Gc-globulin) to human chromosome 4q11-q13 and identification of an associated DNA polymorphism

  • Nancy E. CookeAffiliated withDepartment of Medicine, University of PennsylvaniaDepartment of Human Genetics, University of Pennsylvania
  • , H. F. WillardAffiliated withDepartment of Medical Genetics, University of Toronto
  • , E. V. DavidAffiliated withDepartment of Medicine, University of PennsylvaniaDepartment of Human Genetics, University of Pennsylvania
  • , Donna L. GeorgeAffiliated withDepartment of Human Genetics, University of PennsylvaniaHoward Hughes Medical Institute, University of Pennsylvania

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Summary

Using a characterized human vitamin D binding protein (DBP) cDNA probe and a panel of rodent X human somatic cell hybrids, we established the chromosomal location of the structural gene for DBP on human chromosome 4. In situ hybridization of 3H-labeled DBP cDNA to human metaphase chromosomes confirmed this assignment and allowed regional localization to bands 4q11–4q13. A restriction fragment length polymorphism associated with the DBP gene that should prove useful in future linkage studies was identified with the enzyme BamHI.