Antonie van Leeuwenhoek

, Volume 42, Issue 1, pp 145–155

Autolysis of high-GC isolates of Pseudomonas putrefaciens

  • R. E. Levin
  • Camilla Van Sickle

DOI: 10.1007/BF00399459

Cite this article as:
Levin, R.E. & Van Sickle, C. Antonie van Leeuwenhoek (1976) 42: 145. doi:10.1007/BF00399459


High-GC isolates of P. putrefaciens undergo extensive autolysis after growth, resulting in a marked decrease in turbidity and the release of high-molecular-weight DNA which imparts a high viscosity to culture broths. The native DNA released is resistant to attack by the exocellular DNase activity of the culture broths. Autolysis is inhibited by a pH of 6.0 and the presence of 0.001 m Mg++ or Ca++, and is enhanced by elevated pH values and temperatures. This autolytic phenomenon in broth cultures readily distinguishes high- from low-GC isolates. The latter do not exhibit autolysis.

Copyright information

© H. Veenman en Zonen 1976

Authors and Affiliations

  • R. E. Levin
    • 1
  • Camilla Van Sickle
    • 1
  1. 1.Department of Food Science and NutritionUniversity of MassachusettsAmherstU.S.A.

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