Physiology and Growth

Antonie van Leeuwenhoek

, Volume 47, Issue 1, pp 11-24

First online:

Induction by glucose of an antimycin-insensitive, azide-sensitive respiration in the yeast Kluyveromyces lactis

  • Iliana FerreroAffiliated withIstituto di Genetica, Università di Parma
  • , Anna-Maria ViolaAffiliated withIstituto di Genetica, Università di Parma
  • , A. GoffeauAffiliated withLaboratoire d'Enzymologie, Université Catholique de Louvain

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Increasing the glucose concentration from 0.1 to 10% in exponentially growing cultures of Kluyveromyces lactis CBS 2359 does not repress the antimycin-sensitive respiration (QO 2 of 80 μl O2·h-1·mg-1 dry weight) but raises the antimycin-insensitive respiration from 3 to 12 μl O2·h-1·mg-1 dry weight. Antimycin A inhibits the growth of K. lactis on a variety of substrates with the exception of glucose at concentrations equal to or higher than 1% where substantial antimycin-insensitive respiratory rates are induced. It can be concluded that a minimal antimycin-insensitive QO 2 is necessary for cellular growth when the normal respiratory pathway is not functional.

The antimycin-insensitive respiration elicited by growth in high glucose concentrations is poorly inhibited by hydroxamate and is inhibited by 50% by 90 μm azide or 1mm cyanide. These concentrations are much higher than those necessary to inhibit cytochrome c oxidase which is not involved in the antimycin-insensitive respiration as was demonstrated by spectral measurements. A pigment absorbing at 555 nm is specifically reduced after addition of glucose to antimycin-inhibited cells. The same pigment is reoxidized by further addition of high concentrations of sodium azide indicating its participation in the antimycin-insensitive, azide-sensitive respiration.