Transport of basic amino acids in Riccia fluitans: Evidence for a second binding site Article Received: 12 March 1985 Accepted: 30 April 1985 DOI:
10.1007/BF00397355 Cite this article as: Johannes, E. & Felle, H. Planta (1985) 166: 244. doi:10.1007/BF00397355 Abstract
The transport of several amino acids with different side-chain characteristics has been investigated in the aquatic liverwort
Riccia fluitans. i) The saturation of system I (neutral amino acids) by addition of excess α-aminoisobutyric acid to the external medium completely eliminated the electrical effects which are usually set off by neutral amino acids. Under these conditions arginine and lysine significantly depolarized the plasmalemma. ii) L- and D-lysine/arginine were discriminated against in favour of the L-isomers. iii) Increasing the external proton concentration in the interval pH 9 to 4.5 stimulated plasmalemma depolarization, electrical net current, and uptake of [ 14C]-basic amino acids. iv) Uptake of [ 14C]-glutamic acid took place only at acidic pHs. v) [ 14C]-histidine uptake had an optimum between pH 6 and 5.5. vi) Overlapping of the transport of basic, neutral, and acidic amino acids was common. It is suggested that besides system I, a second system (II), specific for basic amino acids, exists in the plasmalemma of Riccia fluitans. It is concluded that the amino-acid molecule with an uncharged side chain is the substrate for system I, which also binds and transports the neutral species of acidic amino acids, whereas system II is specific for amino acids with a positively charged side chain. The possibility of system II being a proton cotransport is discussed. Key words Amino acid (basic, transport) Bryophyta Membrane depolarization Proton cotransport Riccia Abbreviation AiB
Bertl, A., Felle, H., Bentrup, F.W. (1984) Amine transport in
. Cytoplasmic and vacuolar pH recorded by a pH-sensitive microelectrode. Plant Physiol.
Cheung, Y.-N.S., Nobel, P.S. (1973) Amino acid uptake by pea leaf fragments. Specificity, energy sources, and mechanism. Plant Physiol.
Cole, K.S. (1968) Membrane, ions and impulses. pp. 152–168, University of California Press, Berkeley
Christensen, H.N., Handlogten, M.E. (1969) Reactions of neutral amino acids plus Na
with a cationic amino acid transport system. FEBS Lett.
Felle, H. (1980) Amine transport at the plasmalemma of
. Biochim. Biophys. Acta
Felle, H. (1981a) Sterospecificity and electrogenicity of amino acid transport in
Felle, H. (1981b) A study of the current-voltage relationships of electrogenic active and passive membrane elements in
. Biochim. Biophys. Acta
Felle, H. (1983) Driving forces and current-voltage characteristics of amino acid transport in rhizoid cells of
. Is the carrier negatively charged? Biochim. Biophys. Acta
Felle, H., Bentrup, F.W. (1980) Hexose transport and membrane depolarization in
Felle, H., Gogarten, J.P., Bentrup, F.W., (1983) Phlorizin inhibits hexose transport across the plasmalemma of
Felle, H., Lühring, H., Bentrup, F.W. (1979) Serine transport and membrane depolarization in the liverwort
. Z. Naturforsch. Teil C
Hüsemann, W., Barz, W. (1977) Photoautotrophic growth and photosynthesis in cell suspension cultures from
. Physiol. Plant.
Jung, K.-D., Lüttge, U. (1980) Amino acid uptake by
by a mechanism with affinity to neutral
-amino acids. Planta
Kinraide, T.B., Etherton, B. (1980) Electrical evidence for different mechanisms of uptake for basic, neutral, and acidic amino acids in oat coleoptiles. Plant Physiol.
Kinraide, T.B., Newman, I.A., Etherton, B. (1984) A quantitative simulation model for H
-amino acid cotransport to interpret the effects of amino acids on membrane potential and extracellular pH. Plant Physiol.
Komor, E., Tanner, W. (1971) Characterization of the active hexose transport system of
. Biochim. Biophys. Acta
Komor, E., Tanner, W. (1974) The hexose-proton cotransport system of
: pH-dependent change in K
values and translocation constants of the uptake system. J. Gen. Physiol.
Mitchell, P. (1963) Molecule, group and electron translocation through natural membranes. Biochem. Soc. Symp.
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco cultures. Physiol. Plant.
Reinhold, L., Shtarkshall, R.A., Ganot, D. (1970) Transport of amino acids in barley leaf tissue. II. The kinetics of an unnatural analogue. J. Exp. Bot.
Sanders, D., Slayman, C.L., Pall, M.L. (1983) Stoichiometry of H
/amino acid cotransport in
revealed by current-voltage analysis. Biochim. Biophys. Acta
Sanders, D., Hansen, U.-P., Gradmann, D., Slayman, C.L. (1984) Generalized kinetic analysis of selective ionic effects on Michaelis parameters. J. Membrane Biol.
Sauer, N. (1984) A general amino acid permease is inducible in
Steinmüller, F., Bentrup, F.W. (1981) Amino acid transport in photoautotrophic suspension cells of
L.: Stereospecificity and interaction with potassium ions. Z. Pflanzenphysiol.
von Bel, J.E.A., van Erven, A. (1979) Potassium cotransport and antiport during the uptake of sucrose and glutamic acid from the xylem vessels. Plant Sci. Lett.