Planta

, Volume 175, Issue 4, pp 452–459

Glycine decarboxylase is confined to the bundle-sheath cells of leaves of C3−C4 intermediate species

  • Christopher M. Hylton
  • Stephen Rawsthorne
  • Alison M. Smith
  • D. Alan Jones
  • Harold W. Woolhouse
Article

DOI: 10.1007/BF00393064

Cite this article as:
Hylton, C.M., Rawsthorne, S., Smith, A.M. et al. Planta (1988) 175: 452. doi:10.1007/BF00393064

Abstract

Immunogold labelling has been used to determine the cellular distribution of glycine decarboxylase in leaves of C3, C3−C4 intermediate and C4 species in the genera Moricandia, Panicum, Flaveria and Mollugo. In the C3 species Moricandia foleyi and Panicum laxum, glycine decarboxylase was present in the mitochondria of both mesophyll and bundle-sheath cells. However, in all the C3−C4 intermediate (M. arvensis var. garamatum, M. nitens, M. sinaica, M. spinosa, M. suffruticosa, P. milioides, Flaveria floridana, F. linearis, Mollugo verticillata) and C4 (P. prionitis, F. trinervia) species studied glycine decarboxylase was present in the mitochondria of only the bundle-sheath cells. The bundle-sheath cells of all the C3−C4 intermediate species have on their centripetal faces numerous mitochondria which are larger in profile area than those in mesophyll cells and are in close association with chloroplasts and peroxisomes. Confinement of glycine decarboxylase to the bundle-sheath cells is likely to improve the potential for recapture of photorespired CO2 via the Calvin cycle and could account for the low rate of photorespiration in all C3−C4 intermediate species.

Key words

C4 evolution C3−C4 intermediate plants Flaveria Glycine decarboxylase (localization) Photosynthesis (C3−C4 intermediate) Photorespiration 

Abbreviation and symbol

kDa

kilodaltons

Γ

CO2 compensation point

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Christopher M. Hylton
    • 1
    • 2
  • Stephen Rawsthorne
    • 1
    • 2
  • Alison M. Smith
    • 1
    • 2
  • D. Alan Jones
    • 1
    • 2
  • Harold W. Woolhouse
    • 1
    • 2
  1. 1.John Innes InstituteNorwichUK
  2. 2.AFRC Institute of Plant Science ResearchNorwichUK