Planta

, Volume 156, Issue 6, pp 579–582

Temperature dependence of intracellular pH in higher plant cells

A 31P nuclear magnetic resonance study on maize root tips

Authors

  • P. Aducci
    • Gruppo di Chimica Biologica e Strutturistica Chimica, Instituto di Chimica BiologicaUniversità di Roma
  • R. Federico
    • Gruppo di Chimica Biologica e Strutturistica Chimica, Instituto di Chimica BiologicaUniversità di Roma
  • G. Carpinelli
    • Laboratorio di biologia Cellulare ed ImmunologiaIstituto Superiore di Sanità
  • F. Podo
    • Laboratorio di biologia Cellulare ed ImmunologiaIstituto Superiore di Sanità
Article

DOI: 10.1007/BF00392784

Cite this article as:
Aducci, P., Federico, R., Carpinelli, G. et al. Planta (1982) 156: 579. doi:10.1007/BF00392784

Abstract

The recent introduction of 31P nuclear magnetic resonance spectroscopy offers a new approach to the problem of obtaining a simultaneous and direct evaluation of both the cytoplasmic and vacuolar pH in higher plant cells (J. K. M. Roberts, P.M. Ray, N. Waderlardetzky and O. Sardetzky, 1980, Nature 283, 870–872; 1981, Planta 152, 74–78). Using this method we have been able to detect a selective pH decrease of about 0.5 units at the level of the cytoplasmic compartment of maize root tips when the temperature was increased from 4 to 28°C. This effect was completely reversible with temperature. No pH variation could be detected at the level of the vacuolar compartment.

Key words

pH, intracellularRoot tipZea (pH)

Copyright information

© Springer-Verlag 1982