Cytoplasmic free calcium in Riccia fluitans L. and Zea mays L.: Interaction of Ca 2+ and pH? Article Received: 10 February 1988 Accepted: 19 May 1988 DOI:
Cite this article as: Felle, H. Planta (1988) 176: 248. doi:10.1007/BF00392452 Abstract
In cells of
Zea mays (root hairs, coleoptiles) and Riccia fluitans (rhizoids, thalli) intracellular Ca 2+ and pH have been measured with double-barrelled microelectrodes. Free Ca 2+ activities of 109–187 nM ( Riccia rhizoids), 94–160 nM ( Riccia thalli), 145–231 nM ( Zea root hairs), 84–143 nM ( Zea coleoptiles) were found, and therefore identified as cytoplasmic. In a few cases ( Riccia rhizoids), free Ca 2+ was in the lower millimolar range (2.3±0.8 mM). A change in external Ca 2+ from 0.1 to 10 mM caused an initial and short transient increase in cytoplasmic free Ca 2+ which finally levelled off at about 0.2 pCa unit below the control, whereas in the presence of cyanide the Ca 2+ activity returned to the control level. It is suggested that this behaviour is indicative of active cellular Ca 2+ regulation, and since it is energy-dependent, may involve a Ca 2+-ATPase. Acidification of the cytoplasmic pH and alkalinization of the vacuolar pH lead to a simultaneous increase in cytoplasmic free Ca 2+, while alkalinization of pH c decreased the Ca 2+ activity. Since this is true for such remote organisms as Riccia and Zea, it may be concluded that regulation of cytoplasmic pH and free Ca 2+ are interrelated. It is further concluded that double-barrelled microelectrodes are useful tools for investigations of intracellular ion activities in plant cells. Key words Calcium (free, and pH) Cytoplasm (free Ca 2+, pH) Microelectrode, double-barrelled, Ca 2+ pH(interaction with Ca 2+) Riccia Zea (free Ca 2+ and pH) Symbols and abbreviations Ψ m, ΔΨ m
membrane potential difference, changes thereof
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