Planta

, Volume 178, Issue 3, pp 385–392

The degrees of polymerization and N-acetylation of chitosan determine its ability to elicit callose formation in suspension cells and protoplasts of Catharanthus roseus

Authors

  • H. Kauss
    • Fachbereich BiologieUniversität Kaiserslautern
  • W. Jeblick
    • Fachbereich BiologieUniversität Kaiserslautern
  • A. Domard
    • Laboratoire des matériaux plastiques et des biomatèriauxUniversité Claude Bernard
Article

DOI: 10.1007/BF00391866

Cite this article as:
Kauss, H., Jeblick, W. & Domard, A. Planta (1989) 178: 385. doi:10.1007/BF00391866

Abstract

Partially and fully deacetylated chitosan fragments and oligomers were compared for their potency to elicit formation of the 1.3-β-glucan callose in suspension-cultured cells and protoplasts of Catharanthus roseus (line 385). Chitosan oligomers induced little callose formation, while callose synthesis increased with the degree of polymerization of chitosan up to several thousand corresponding to a molecular mass near 106 Da. At a comparable degree of polymerization, partially N-acetylated chitosan fragments were less effective. Colloidal chitin and chitin oligomers induced only trace callose synthesis in protoplasts. These results indicate that the primary interaction involved the amino groups of chitosan and numerous negative charges at the surface of the plasma membrane with spacing in the nanometer range and occurring regularly over micrometer stretches. Charged phospholipid head-groups may fulfill these requirements. The resulting alteration of membrane fluidity may lead to the changes in ion transport known to be associated with the induction of callose formation.

Key words

Calcium (callose induction)Callose induction (chitosan)Catharanthus (callose induction)Chitosan (N-acetylation, oligomers)Plasma membrane (callose induction)

Abbreviations

DP

degree of polymerization

FDA

fluorescein diacetate

PE

pachyman equivalents

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Copyright information

© Springer-Verlag 1989