Molecular and General Genetics MGG

, Volume 220, Issue 3, pp 441–447

A single treatment of rice seedlings with 5-azacytidine induces heritable dwarfism and undermethylation of genomic DNA

Authors

  • Hiroshi Sano
    • Laboratory of Molecular Genetics, Biotechnology InstituteAkita Prefectural College of Agriculture
  • Ikuko Kamada
    • Laboratory of Molecular Genetics, Biotechnology InstituteAkita Prefectural College of Agriculture
  • Shohab Youssefian
    • Laboratory of Molecular Genetics, Biotechnology InstituteAkita Prefectural College of Agriculture
  • Masayuki Katsumi
    • Department of BiologyInternational Christian University
  • Hiroetsu Wabiko
    • Laboratory of Molecular Genetics, Biotechnology InstituteAkita Prefectural College of Agriculture
Article

DOI: 10.1007/BF00391751

Cite this article as:
Sano, H., Kamada, I., Youssefian, S. et al. Mol Gen Genet (1990) 220: 441. doi:10.1007/BF00391751

Summary

A single exposure of germinated rice seeds (Oryza sativa) to either of the DNA demethylating agents 5-azacytidine (azaC) or 5-azadeoxycytidine (azadC) induced dwarf plants. At maturity, seeds treated with azaC exhibited normal morphological characteristics in comparison with untreated controls except that their height (total stem length) was reduced by about 15%. The M1 progeny, obtained by self-fertilization of an azaC-induced dwarf plant, segregated into dwarf (35%) and apparently tall types (65%). The M2 progenies, obtained by self-fertilization of dwarf M1 plants, were also dwarf, while those from tall M1 plants were only tall. Genomic DNA isolated from mature leaves of azaC-treated seeds showed about a 16% reduction in the 5-methylcytosine (m5C) content in comparison with DNA from untreated samples. A similar reduction in the m5C content was also observed in the M1 and M2 progenies. Thus, both undermethylation and dwarfism induced by azaC treatment were heritable. The results suggest that azaC induced demethylation of genomic DNA, which caused an altered pattern of gene expression and consequently a reduction in plant stem length.

Key words

5-AzacytidineDNA methylationDwarf plantsOryza sativa

Copyright information

© Springer-Verlag 1990