Planta

, Volume 167, Issue 2, pp 300–302

Occurrence of diamine oxidase in the apoplast of pea epicotyls

  • R. Federico
  • R. Angelini
Short Communication

DOI: 10.1007/BF00391430

Cite this article as:
Federico, R. & Angelini, R. Planta (1986) 167: 300. doi:10.1007/BF00391430

Abstract

Most of the diamine oxidase (EC 1.4.3.6) present in pea (Pisum sativum L. cv. Rondo) epicotyls is found in the fluid obtained by centrifuging pea epicotyl sections previously infiltrated under vacuum with a buffer solution. No detectable amount of the cytoplasmic enzyme glucose-6-phosphate dehydrogenase is present in this fluid, showing that there is very little contamination by cell contents. Polyacrylamide-gel electrophoresis and specific-activity data indicate that diamine oxidase is the most plentiful protein in the extracellular solution obtained from pea epicotyl sections and that an active process is involved in the selective transfer of the enzyme outside the cell. The possible involvement of diamine oxidase in the supply of H2O2 to peroxidase-catalyzed reactions occurring inside the cell wall is discussed.

Key words

Diamine oxidase Extracellular solution Hydrogen peroxide production Pisum (diamine oxidase) 

Abbreviations

DAO

diamine oxidase

Glc6P

glucose-6-phosphate

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • R. Federico
    • 1
  • R. Angelini
    • 1
  1. 1.Dipartimento di Biologia VegetaleUniversità “La Sapienza”RomaItalia

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