Current Genetics

, Volume 11, Issue 1, pp 71–77

Large mitochondrial genome and mitochondrial DNA size polymorphism in the mosquito parasite, Romanomermis culicivorax

  • Thomas O. Powers
  • E. G. Plazzer
  • Bradley C. Hyman
Original Article

DOI: 10.1007/BF00389428

Cite this article as:
Powers, T.O., Plazzer, E.G. & Hyman, B.C. Curr Genet (1986) 11: 71. doi:10.1007/BF00389428

Summary

Physical characterization of the mitochondrial genome derived from the obligate mosquito parasite, Romanomermis culicivorax has generated some surprising physical properties regarding the molecular structure of nematode mitochondrial DNA (mtDNA). Restriction enzyme analysis of this mtDNA has revealed a mitochondrial genome size of approximately 26 kb, the largest metazoan mtDNA reported to date. Isofemale lineages are monomorphic for one of three size variants, differing by 500-1,000 base pairs, present in our original field population. Cloned hybridization probes derived from a single region exhibiting a 600 by size polymorphism share strong homology with several spatially separated sites distributed about the mtDNA. This suggests that the homology is a result of repeated DNA sequence elements contained within this mitochondrial genome that contribute to mtDNA size polymorphism.

Key words

Mitochondrial DNAPopulation heterogeneityMolecular evolutionRecombinant DNA

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Thomas O. Powers
    • 1
  • E. G. Plazzer
    • 1
  • Bradley C. Hyman
    • 2
  1. 1.Department of NematologyUniversity of CaliforniaRiversideUSA
  2. 2.Department of BiologyUniversity of CaliforniaRiversideUSA
  3. 3.Department of Plant PathologyUniversity of NebraskaLincolnUSA