Planta

, Volume 145, Issue 2, pp 129–135

Fractionation of particulate material from maize coleoptile homogenates with polyethylene glycol

  • Wolfgang Michalke
  • Brigitte Schmieder
Article

DOI: 10.1007/BF00388708

Cite this article as:
Michalke, W. & Schmieder, B. Planta (1979) 145: 129. doi:10.1007/BF00388708

Abstract

Polyethyleneglycol (PEG) has been used to sediment particulate material from maize coleoptile homogenates at low centrifugal forces. The resuspended sediments were used for N-1-naphthylphthalamic acid (NPA)-binding studies. Binding activity was influenced by monovalent cations in the resuspension medium, but even at concentrations of up to 1.2 M NaCl or 0.5 M LiCl or CsCl, half of the binding activity was still recovered. Binding activity was influenced by divalent cations, because it decreased when Ca2+ and Mg2+ ions in the medium were complexed with EDTA. Fractionated sedimentation using increasing concentrations of PEG resulted in two peaks of NPA-binding activity at about 3% and 6% PEG. The 3% peak cintained enzymatic markers for mitochondria and endoplasmatic reticulum while the 6% peak contained NPA-binding activity only. Possible explanations for the bimodal distribution of NPA binding after fractionated PEG precipitation are discussed.

Key words

Coleoptile Naphthylphthalamic acid binding Polyethylene glycol Zea 

Abbreviations

IAA

indoleacetic acid

KF

see Introduction

NAA

naphthylacetic acid

NPA

N-1-naphthylphthalamic acid

PEG

polyethyleneglycol

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Wolfgang Michalke
    • 1
  • Brigitte Schmieder
    • 1
  1. 1.Institut für Biologie III der Universität FreiburgFreiburgFederal Republic of Germany