Human Genetics

, Volume 71, Issue 4, pp 325–328

Human ferritin light chain gene sequences mapped to several sorted chromosomes

Authors

  • R. V. Lebo
    • Howard Hughes Medical Institute and Department of MedicineUniversity of California
  • Y. W. Kan
    • Howard Hughes Medical Institute and Department of MedicineUniversity of California
  • Mei-Chi Cheung
    • Howard Hughes Medical Institute and Department of MedicineUniversity of California
  • S. K. Jain
    • Department of Biochemistry and PharmacologyTufts Medical School
  • J. Drysdale
    • Department of Biochemistry and PharmacologyTufts Medical School
Original Investigations

DOI: 10.1007/BF00388458

Cite this article as:
Lebo, R.V., Kan, Y.W., Cheung, M. et al. Hum Genet (1985) 71: 325. doi:10.1007/BF00388458

Summary

The iron storage ferritin light-chain gene exhibits multiple restriction enzyme fragments which have been mapped by analyzing sorted human chromosomes. A dual laser chromosome sorter was used to construct spot-blot filter panels representing 22 chromosome fractions. Hybridization of radiolabeled human ferritin-L gene probe to spot-blot panels revealed the ferritin-L gene on more than one chromosome. Miniaturized restriction enzyme analysis was used to map each of the ferritin-L restriction fragments uniquely to one of three chromosomes. This combination of sorted chromosome analyses provides a rapid method to map homologous DNA sequences located on more than one chromosome.

Copyright information

© Springer-Verlag 1985