Original Investigations

Human Genetics

, Volume 71, Issue 4, pp 325-328

First online:

Human ferritin light chain gene sequences mapped to several sorted chromosomes

  • R. V. LeboAffiliated withHoward Hughes Medical Institute and Department of Medicine, University of California
  • , Y. W. KanAffiliated withHoward Hughes Medical Institute and Department of Medicine, University of California
  • , Mei-Chi CheungAffiliated withHoward Hughes Medical Institute and Department of Medicine, University of California
  • , S. K. JainAffiliated withDepartment of Biochemistry and Pharmacology, Tufts Medical School
  • , J. DrysdaleAffiliated withDepartment of Biochemistry and Pharmacology, Tufts Medical School

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Summary

The iron storage ferritin light-chain gene exhibits multiple restriction enzyme fragments which have been mapped by analyzing sorted human chromosomes. A dual laser chromosome sorter was used to construct spot-blot filter panels representing 22 chromosome fractions. Hybridization of radiolabeled human ferritin-L gene probe to spot-blot panels revealed the ferritin-L gene on more than one chromosome. Miniaturized restriction enzyme analysis was used to map each of the ferritin-L restriction fragments uniquely to one of three chromosomes. This combination of sorted chromosome analyses provides a rapid method to map homologous DNA sequences located on more than one chromosome.