Quantitative ion localization within Suaeda maritima leaf mesophyll cells
- Cite this article as:
- Harvey, D.M.R., Hall, J.L., Flowers, T.J. et al. Planta (1981) 151: 555. doi:10.1007/BF00387435
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Grown under saline conditions, Suaeda maritima accumulates Na+ and Cl- into its leaves, where individual mesophyll cells behave differently in their compartmentation of these ions. Measurements of ion concentrations within selected subcellular compartments show that freeze-substitution with dry sectioning is a valuable preparative technique for analytical electron microscopy of highly vacuolate plant material. Using this approach, absolute estimates were made of Na+, K+ and Cl- concentrations in the cytoplasm, cell walls, chloroplasts and vacuoles of leaf mesophyll cells.
Key wordsHalophyteIon accumulationSuaeda
transmission analytical electron microscopy