, Volume 98, Issue 1, pp 89-96

Physiological aspects of conjugation in fission yeast

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Summary

Conjugation was studied in Schizosaccharomyces pombe using liquid media. Nitrogen, which was growth-limiting in a synthetic medium, had to be consumed completely before conjugation could start. Conjugation was preceded by sexual agglutination. Agglutinability was not constitutive in heterothallic strains. It only developed when cells of h + and h - mating type were grown in mixed culture for at least 2.5 hr before the start of conjugation.