Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study
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Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. The distribution based on small-angle light scattering is similar to the distribution based on volume as measured by Elzone particle counter. It thus appears that viable cells form the population with a larger mean cell volume. The results also indicate that the volume of viable cells decreases during the cultivation while that of dead cells remains relatively constant.
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- Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study
Volume 2, Issue 2 , pp 85-94
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- Kluwer Academic Publishers
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- cell volume
- cell culture
- flow cytometry
- Industry Sectors
- Author Affiliations
- 1. Department of Chemical Engineering and Materials Science, University of Minnesota, 55455, Minneapolis, MN, USA
- 2. Institute for Advanced Studies in Biological Process Technology, University of Minnesota, 55108, St. Paul, MN, USA