Cytotechnology

, Volume 2, Issue 2, pp 85–94

Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study

Authors

  • Sankar Sen
    • Department of Chemical Engineering and Materials ScienceUniversity of Minnesota
  • Friedrich Srienc
    • Department of Chemical Engineering and Materials ScienceUniversity of Minnesota
    • Institute for Advanced Studies in Biological Process TechnologyUniversity of Minnesota
  • Wei-Shou Hu
    • Department of Chemical Engineering and Materials ScienceUniversity of Minnesota
Review

DOI: 10.1007/BF00386140

Cite this article as:
Sen, S., Srienc, F. & Hu, W. Cytotechnology (1989) 2: 85. doi:10.1007/BF00386140

Abstract

Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. The distribution based on small-angle light scattering is similar to the distribution based on volume as measured by Elzone particle counter. It thus appears that viable cells form the population with a larger mean cell volume. The results also indicate that the volume of viable cells decreases during the cultivation while that of dead cells remains relatively constant.

Key words

hybridomacell volumecell cultureflow cytometry

Copyright information

© Kluwer Academic Publishers 1989