Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study
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- Sen, S., Srienc, F. & Hu, WS. Cytotechnology (1989) 2: 85. doi:10.1007/BF00386140
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Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. The distribution based on small-angle light scattering is similar to the distribution based on volume as measured by Elzone particle counter. It thus appears that viable cells form the population with a larger mean cell volume. The results also indicate that the volume of viable cells decreases during the cultivation while that of dead cells remains relatively constant.