Current Genetics

, Volume 11, Issue 6, pp 513–519

Biased inheritance of optional insertions following mitochondrial genome recombination in the basidiomycete fungus Coprinus cinereuss

  • A. Economou
  • V. Lees
  • P. J. Pukkila
  • M. E. Zolan
  • L. A. Casselton
Original Articles

DOI: 10.1007/BF00384614

Cite this article as:
Economou, A., Lees, V., Pukkila, P.J. et al. Curr Genet (1987) 11: 513. doi:10.1007/BF00384614

Summary

Two mitochondrial genomes of Coprinus cinereus, H and J, were found to have alternative 1.23 kb insertions. Using the Neurospora crassa cytochrome oxidase-1 (co-1) gene as a probe, the “J” insertion site was shown to be located within the Coprinus co-1 gene, whereas the “H” insertion was some 2 kb distant. The insertions showed biased inheritance following mitochondrial genome recombination. Recombination between H and J genomes was detected using the mitochondrial gene mutations acu-10, which causes a cytochrome oxidase defect, and cap-1, which confers chloramphenicol resistance. Fourteen of fifteen independently derived recombinants for these two genes were shown to have both DNA insertions. In a second series of H x J crosses, intragenic recombination between different cap-1 alleles was detected. These mutations are assumed to be in the large ribosomal RNA gene some 6 kb distant from the nearest insertion site. Each of eight independently derived cap-1+ recombinants had both DNA insertions. Despite their similar size and similar behaviour following recombination the insertions do not share extensive sequence homology.

Key words

MitochondriaMitochondrial insertionsUnidirectional conversionCoprinus

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • A. Economou
    • 1
  • V. Lees
    • 1
  • P. J. Pukkila
    • 2
  • M. E. Zolan
    • 2
  • L. A. Casselton
    • 1
  1. 1.School of Biological SciencesQueen Mary CollegeLondonUK
  2. 2.Department of Biology and Curriculum in GeneticsUniversity of North Carolina at Chapel HillChapel HillUSA