Expression of storage-protein genes during soybean seed development
- Cite this article as:
- Meinke, D.W., Chen, J. & Beachy, R.N. Planta (1981) 153: 130. doi:10.1007/BF00384094
Mature seeds of Glycine max (L.) Merr. contain two major storage proteins, a glycosylated 7S protein (conglycinin) and a non-glycosylated 11S protein (glycinin). Accumulation of these proteins and their mRNAs during seed development in cv. Provar was studied by SDS polyacrylamide gel electrophoresis and by “Northern” (DNA-RNA) hybridization. The 11S acidic and basic subunits and the 7S α′ and α subunits began to accumulate 18–20 d after pollination, shortly after the termination of cell division in developing cotyledons, whereas the 7S β and 11S A-4 subunits were not detected until one to two weeks later, during the maturation phase of development. Messenger RNAs for 7S and 11S proteins were first detected 14–18 d after pollination, several days before the accumulation of storage proteins. Extracts from embryonic axes contained reduced levels of the 7S α subunit, very little 11S protein, no detectable 7S β or 11S A-4 subunits, and an additional 7S subunit not found in cotyledons. Soybean axes and cotyledons therefore differ in their synthesis of seed storage proteins.
Key wordsEmbryonic axisGlycinemRNA accumulationSeed developmentStorage proteins
sodium dodecyl sulfate