Roux's archives of developmental biology

, Volume 198, Issue 6, pp 330–335

Mesodermal induction in early amphibian embryos by activin A (erythroid differentiation factor)

  • Makoto Asashima
  • Hiroshi Nakano
  • Kazunori Shimada
  • Kei Kinoshita
  • Koichi Ishii
  • Hiroshiro Shibai
  • Naoto Ueno
Article

DOI: 10.1007/BF00383771

Cite this article as:
Asashima, M., Nakano, H., Shimada, K. et al. Roux's Arch Dev Biol (1990) 198: 330. doi:10.1007/BF00383771

Summary

Recently the mesoderm-inducing effects of the transforming growth factor β (TGF-β) family of proteins have been widely examined. In an attemt to elucidate the functions of these proteins, porcine inhibin A and activin A (erythroid differentiation factor; EDF) were examined. Treatment of explants with activin A led to differentiation of mesodermal derivatives such as mesenchyme, notochord, blood cells and muscle, but inhibin A had a much lesser effect. The mesodermal differentiation induced by activin A was also comfirmed by analyses using a polyclonal antibody against muscle myosin. By indirect immunofluorescence analysis, the differentiation of muscle blocks was observed in the activin-A-treated explants, whereas no differentiation was observed in inhibin-A-treated and control explants. These findings confirm that this protein of the TGF-β family has mesoderm-inducing ability.

Key words

Mesoderm inductionActivin AErythroid differentiation factorXenopus laevis

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Makoto Asashima
    • 1
  • Hiroshi Nakano
    • 1
  • Kazunori Shimada
    • 1
  • Kei Kinoshita
    • 2
  • Koichi Ishii
    • 3
  • Hiroshiro Shibai
    • 3
  • Naoto Ueno
    • 4
  1. 1.Department of BiologyYokohama City UniversityYokohamaJapan
  2. 2.Biological LaboratoryNippon Medical SchoolKawasakiJapan
  3. 3.Central Research LaboratoriesAjinomoto Co. Inc.KawasakiJapan
  4. 4.Institute of Applied BiochemistryTsukuba UniversityIbarakiJapan