, Volume 200, Issue 1, pp 65-67

Transposon donor plasmids, based on ColIb-P9, for use in Pseudomonas putida and a variety of other Gram negative bacteria

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Summary

The properties of pLG221, a derivative of the ColIb plasmid carrying the transposon Tn5 are described. This plasmid can be used to introduce Tn5 by conjugation from Escherichia coli into a variety of Gram negative bacteria outside the host range for maintenance of ColIb. Plasmid pLG221, and a similar plasmid pLG223 carrying Tn10 may be of general utility as vectors for transposon-mediated mutagenesis in a variety of Gram negative bacteria.

Communicated by P.T. Emmerson