Molecular and General Genetics MGG

, Volume 193, Issue 3, pp 437–444

Arginine degradation in Pseudomonas aeruginosa mutants blocked in two arginine catabolic pathways

Authors

  • Dieter Haas
    • Mikrobiologisches InstitutEidgenössische Technische Hochschule
  • Hideki Matsumoto
    • Department of Bacteriology, School of MedicineShinshu University
  • Paola Moretti
    • Mikrobiologisches InstitutEidgenössische Technische Hochschule
  • Victor Stalon
    • Laboratoire de Microbiologie, Faculté des SciencesUniversité Libre de Bruxelles
  • Annick Mercenier
    • Laboratoire de Microbiologie, Faculté des SciencesUniversité Libre de Bruxelles
Article

DOI: 10.1007/BF00382081

Cite this article as:
Haas, D., Matsumoto, H., Moretti, P. et al. Molec. Gen. Genet. (1984) 193: 437. doi:10.1007/BF00382081

Summary

Pseudomonas aeruginosa mutants defective in agmatine utilization (agu) were isolated. The genes encoding agmatine deiminase (aguA) and N-carbamoylputrescine amidinohydrolase (aguB) were 98% cotransducible and mapped between gpu and ser-3 in the 30 min region of the chromosome. Constructed agu arc double mutants (blocked in the arginine decarboxylase and arginine deiminase pathways) used arginine efficiently as the sole carbon and nitrogen source. This suggests the existence of a further arginine catabolic pathway in P. aeruginosa. The mapping data of this study confirm that in P. aeruginosa the chromosomal genes with catabolic functions do not show supraoperonic clustering as found in P. putida.

Copyright information

© Springer-Verlag 1984