Article

Molecular and General Genetics MGG

, Volume 193, Issue 3, pp 437-444

First online:

Arginine degradation in Pseudomonas aeruginosa mutants blocked in two arginine catabolic pathways

  • Dieter HaasAffiliated withMikrobiologisches Institut, Eidgenössische Technische Hochschule
  • , Hideki MatsumotoAffiliated withDepartment of Bacteriology, School of Medicine, Shinshu University
  • , Paola MorettiAffiliated withMikrobiologisches Institut, Eidgenössische Technische Hochschule
  • , Victor StalonAffiliated withLaboratoire de Microbiologie, Faculté des Sciences, Université Libre de Bruxelles
  • , Annick MercenierAffiliated withLaboratoire de Microbiologie, Faculté des Sciences, Université Libre de Bruxelles

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Summary

Pseudomonas aeruginosa mutants defective in agmatine utilization (agu) were isolated. The genes encoding agmatine deiminase (aguA) and N-carbamoylputrescine amidinohydrolase (aguB) were 98% cotransducible and mapped between gpu and ser-3 in the 30 min region of the chromosome. Constructed agu arc double mutants (blocked in the arginine decarboxylase and arginine deiminase pathways) used arginine efficiently as the sole carbon and nitrogen source. This suggests the existence of a further arginine catabolic pathway in P. aeruginosa. The mapping data of this study confirm that in P. aeruginosa the chromosomal genes with catabolic functions do not show supraoperonic clustering as found in P. putida.