Molecular and General Genetics MGG

, Volume 193, Issue 3, pp 431–436

Revised locations of the hisI and pru (proline utilization) genes on the Pseudomonas aeruginosa chromosome map

Authors

  • Leda Soldati
    • Mikrobiologisches InstitutEidgenössische Technische Hochschule
  • Robert Crockett
    • Department of GeneticsMonash University
  • Judith M. Carrigan
    • Department of GeneticsMonash University
  • Thomas Leisinger
    • Mikrobiologisches InstitutEidgenössische Technische Hochschule
  • Bruce W. Holloway
    • Department of GeneticsMonash University
  • Dieter Haas
    • Mikrobiologisches InstitutEidgenössische Technische Hochschule
Article

DOI: 10.1007/BF00382080

Cite this article as:
Soldati, L., Crockett, R., Carrigan, J.M. et al. Molec. Gen. Genet. (1984) 193: 431. doi:10.1007/BF00382080

Summary

The location of genes in the vicinity of the major FP2 origin on the chromosome of Pseudomonas aeruginosa PAO has been revised. The markers hisI (a transduction group of histidine biosynthetic genes) and pru (a gene cluster encoding proline utilization functions) were located in the 90 to 95/0 min chromosome region by a series of plate matings mediated by R68.45. Three-factor-crosses using this plasmid established the following marker order: pur-67 pru hisI/cys-59 proB ilvB/C. Genetic evidence is presented to confirm the previous observations that FP2 can mobilize the chromosome from at least two origins near proB and in both directions. Thus, when markers in this chromosome region are analyzed by FP2 crosses only, the mapping data may be difficult to interpret. This complication can be overcome by the use of R68.45 and Tfr (transposon-facilitated recombination) or Hfr donors.

Copyright information

© Springer-Verlag 1984