Summary
The ultrastructure of organelles appearing in the early typical and atypical spermatids, and the nutritive cells of Cipangopaludina malleata has been examined by a Siemens' electron microscope Elmiskop I.
Mitochondria appearing in the early typical spermatid have doughnut-like profiles in which the internal ridges appear as triple-layered membranes arranged radially and extending into the interior of the organelle without reaching the other side. Each membrane 40–60 Å in width, separated by a clear interspace 60–90 Å wide, is characterized by a porous structure 20–30 Å in diameter which suggests a filtration apparatus for enzymes.
Walls of the flattened saccules consisting the Golgi apparatus are calculated 35–60 Å thick, in which an electron-lucent, porous structure about 30 Å wide has been revealed.
The smooth-surfaced endoplasmic reticulum is bordered by a triple-layered membrane consisting of two opaque layers with a less opaque interspace 20–30 Å wide. The outer membrane ca. 15 Å wide presents a more linear appearance than the dotted arrangement of the inner membrane 20–25 Å thick.
The plasma membrane is composed of a triple-layered structure where two dense lines 15 Å wide are separated by a layer 20–30 Å thick of less density.
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The electron micrographs for the present studies were taken with the Siemens electron microscope, model Elmiskop I, at the Anatomical Institute of Kiel University, Germany. The one of the authors, G. Yasuzumi is deeply grateful to Prof. Dr. W. Bargmann and Dr. A. Knoop for the privilege of using this instrument and other equipments in the Laboratory.
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Yasuzumi, G., Tanaka, H., Tezuka, O. et al. The ultrastructure of organelles appearing in spermatids and nutritive cells of Cipangopaludina malleata . Z.Zellforsch 50, 632–643 (1959). https://doi.org/10.1007/BF00381675
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DOI: https://doi.org/10.1007/BF00381675