Planta

, Volume 152, Issue 6, pp 562–564

In vitro biosynthesis of the C-glycosidic bond in aloin

  • M. Grün
  • G. Franz
Article

DOI: 10.1007/BF00380828

Cite this article as:
Grün, M. & Franz, G. Planta (1981) 152: 562. doi:10.1007/BF00380828

Abstract

Biosynthetic studies with cell-free extracts from Aloe arborescens Mill. demonstrate the transfer of the glucose moiety from UDP-glucose to aloe emodin anthrone, forming the C-glycosidic linkage in the anthracene derivative aloin. The pH-dependence and the specificity of UDP-glucose and aloe emodin anthrone for the biosynthesis of the C-glycosidic bond in aloin are shown.

Key words

AloeAloinGlucosylation (C-)Glycosides (C-, biosynthesis)

Abbreviations

ADP-Glc

adenosine-5′-diphosphate glucose

AEA

aloe emodin anthrone (1,8-dihydroxy-3-(hydroxymethyl)-9(10 H)-anthracenone)

CoASAc

acetyl coenzyme A

GDP-Glc

guanosine-5′-diphosphate glucose

Glc

glucose

Glc-1-P

glucose-1-phosphate

HPLC

high performance liquid chromatography

TLC

thin layer chromatography

UDP-Gal

uridine-5′-diphosphate galactose

UDP-Glc

uridine-5′-diphosphate glucose

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • M. Grün
    • 1
  • G. Franz
    • 1
  1. 1.Fakultät für Chemie und PharmazieUniversität RegensburgRegensburgFederal Republic of Germany