Current Genetics

, Volume 11, Issue 5, pp 359–367

Temperature sensitive pet mutants in yeast Saccharomyces cerevisiae that lose mitochondrial RNA

  • David M. Mueller
  • Tapan K. Biswas
  • James Backer
  • John C. Edwards
  • Murray Rabinowitz
  • Godfrey S. Getz
Original Articles

DOI: 10.1007/BF00378178

Cite this article as:
Mueller, D.M., Biswas, T.K., Backer, J. et al. Curr Genet (1987) 11: 359. doi:10.1007/BF00378178
  • 53 Downloads

Summary

This is a description of a new class of temperature sensitive pet mutants in Saccharomyces cereviase that lose all or part of their mitochondrial RNA at the restrictive temperature. These mutants fall into 8 different complementation groups, mna1 to mna8, and 2 different classes based on their phenotype. Class I mutations, mna1-1 through mna5-1, cause complete or partial loss of mitochondrial RNA at the restrictive temperature. The mutation, mna1-1, is especially interesting since it causes a loss of both mitochondrial DNA and RNA when the mutant is grown on a fermentable carbon source at the restrictive temperature. However, when this mutant is grown at the permissive temperature on a non-fermentable carbon source then shifted to the restrictive temperature, only the mitochondrial RNA is lost. This indicates that the primary cause for the pet phenotype is due to the loss of mitochondrial RNA and not DNA. Class II mutations, mna6-1 through man8-1, cause complete loss of the 14S rRNA after growth at the restrictive temperature in a fermentable carbon source. This loss appears to be specific for the 14S rRNA, since all other transcripts probed by Northern analysis are normal.

Key words

Yeast Mitochondrial Mutants RNA 

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • David M. Mueller
    • 1
  • Tapan K. Biswas
    • 1
  • James Backer
    • 1
    • 3
  • John C. Edwards
    • 2
  • Murray Rabinowitz
    • 1
    • 2
  • Godfrey S. Getz
    • 1
    • 2
    • 4
  1. 1.Departments of MedicineThe University of ChicagoChicagoUSA
  2. 2.Biochemistry and Molecular BiologyThe University of ChicagoChicagoUSA
  3. 3.Department of Molecular Genetics and BiologyThe University of ChicagoChicagoUSA
  4. 4.Department of PathologyThe University of ChicagoChicagoUSA
  5. 5.Department of Biochemistry and StructureThe Chicago Medical SchoolNorth ChicagoUSA
  6. 6.Department of BiologyConcordia CollegeMoorheadUSA

Personalised recommendations