Current Genetics

, Volume 15, Issue 6, pp 393–398

An α-specific gene, SAG1 is required for sexual agglutination in Saccharomyces cerevisiae

  • Syuichi Doi
  • Kazuyuki Tanabe
  • Masayasu Watanabe
  • Masayoshi Yamaguchi
  • Masao Yoshimura
Original articles

DOI: 10.1007/BF00376793

Cite this article as:
Doi, S., Tanabe, K., Watanabe, M. et al. Curr Genet (1989) 15: 393. doi:10.1007/BF00376793

Summary

Seven α-specific mutants specifically defective in sexual agglutinability were isolated. The other α mating functions exhibited by these mutants, designated sag mutants, such as the production of α pheromone and response to a mating pheromone, were normal. While the MATα sag1 cells did not agglutinate with wild-type a cells, the MATα sag1 cells did, indicating that the SAG1 gene is expressed only in α cells. The mutations were semi-dominant and fell into a single complementation group, SAG1, which was mapped near met3 on chromosome X. Complementation analysis showed that sag1 and aga1, the latter being a previously reported α-specific mutation, were mutations in the same gene.

Key words

YeastMatingSexual agglutinationa-Specific mutation

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Syuichi Doi
    • 1
  • Kazuyuki Tanabe
    • 3
  • Masayasu Watanabe
    • 2
  • Masayoshi Yamaguchi
    • 1
  • Masao Yoshimura
    • 1
  1. 1.Department of Legal MedicineKinki University School of MedicineOsaka-Sayama, OsakaJapan
  2. 2.Department of BacteriologyKinki University School of MedicineOsaka-Sayama, OsakaJapan
  3. 3.Department of Medical ZoologyOsaka City University Medical SchoolAsahi-machi, Abeno-ku, OsakaJapan