Bioprocess Engineering

, Volume 7, Issue 5, pp 235–240

Modelling and simulation of cellulase adsorption and recycling during enzymatic hydrolysis of cellulosic materials

  • J. Bader
  • K. -H. Bellgardt
  • A. Singh
  • P. K. R. Kumar
  • K. Schügerl
Originals

DOI: 10.1007/BF00369552

Cite this article as:
Bader, J., Bellgardt, K.H., Singh, A. et al. Bioprocess Engineering (1992) 7: 235. doi:10.1007/BF00369552

Abstract

Characteristics of cellulase adsorption on cellulosic substrates and their recycling during the hydrolysis of cellulose were studied. On the basis of the experimental data, a comprehensive model was set up comprising of sub-models for the enzyme adsorption, enzymatic hydrolysis and recycling of enzymes. The model equations consist of non-linear systems of ordinary differential and algebraic equations. The model parameters were identified by means of the experimental results of Singh et al. [1] and Bader et al. [2]. The simulation results with the model corresponded well with the experimental data. Thus, the good agreement between simulated and measured process variables indicates that the model is suitable for description of cellulase adsorption and recycling during hydrolysis of cellulosic substrates.

List of Symbols

C g/l

cellulose concentration

CB g/l

cellobiose concentration

cba U/g

specific adsorbed cellobiase

cbam U/g

maximum specific adsorbed cellobiase

cbf U/g

specific free cellobiase

cbt U/g

specific total cellobiase

cea U/g

specific adsorbed cellulase

ena U/g

specific adsorbed endoglucanase

enam U/g

maximum specific adsorbed endoglucanase

enf U/g

specific free endoglucanase

ent U/g

specific total endoglucanase

exa U/g

specific adsorbed exoglucanase

exam U/g

maximum specific adsorbed exoglucanase

exf U/g

specific free exoglucanase

ext U/g

specific total exoglucanase

Exf U/l

free exoglucanase

G g/l

glucose concentration

kc g/U h

rate constant of cellulose hydrolysis

KCB g/l

constant of competitive inhibition by cellobiose (cellulose hydrolysis)

kCB 1/h

rate constant of cellobiose hydrolysis

Kcb U/g

limitation constant of cellobiase adsorption

kcb 1/h

rate constant of cellobiase adsorption

Ken U/g

limitation constant of endoglucanase adsorption

ken 1/h

rate constant of endoglucanase adsorption

Kex U/g

limitation constant of exoglucanase adsorption

kex 1/h

rate constant of exoglucanase adsorption

KG g/l

constant of competitive inhibition by glucose (cellulose hydrolysis)

KICB g/l

constant of competitive inhibition by cellobiose (cellobiose hydrolysis)

KIG g/l

constant of competitive inhibition by glucose (cellobiose hydrolysis)

S g/l

substrate concentration

t h

time

Ucb U/g

specific capacity of cellobiase loading on substrate

Uen U/g

specific capacity of endoglucanase loading on substrate

Uex U/g

specific capacity of exoglucanase loading on substrate

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • J. Bader
    • 1
  • K. -H. Bellgardt
    • 1
  • A. Singh
    • 1
  • P. K. R. Kumar
    • 1
  • K. Schügerl
    • 1
  1. 1.Institut für Technische ChemieUniversität HannoverHannover 1Federal Republic of Germany

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