Uptake, metabolism and discharge of polycyclic aromatic hydrocarbons by marine fish Article DOI:
Cite this article as: Lee, R.F., Sauerheber, R. & Dobbs, G.H. Marine Biology (1972) 17: 201. doi:10.1007/BF00366294 Abstract
The uptake, metabolism and discharge of two polycyclic aromatic hydrocarbons,
14C-naphthalene and 3H-3,4-benzopyrene, were studied in 3 species of marine fish (mudsucker or sand goby, Gillichthys mirabilis; sculpin, Oligocottus maculosus; sand dab, Citharichthys stigmaeus). The path of hydrocarbons through the fish included entrance through the gills, metabolism by the liver, transfer of hydrocarbons and their metabolites to the bile, and, finally, excretion. The gall bladder was a major storage site of labeled hydrocarbons and their metabolites. The major product of 2H-3,4-benzypyrene metabolism was tentatively identified as 7,8-dihydro-7,8-dihydroxybenzopyrene. The 14C-naphthalene was metabolized to 1,2-dihydro-1,2-dihydroxynaphthalene after 24 h exposure. The urine appeared to the major avenne for discharge of labeled hydrocarbon from the body. Our laboratory results indicated that certain polycyelic aromatic hydrocarbons were rapidly taken up from seawater by the above fish, but detoxification mechanisms existed for efficient removal of these compounds from their body tissues.
Communicated by J. Bunt, Miami
Abdel Aziz, F. T., P. C. Hirom, P. Millburn, R. L. Smith and R. T. Williams: The biliary excretion of anions of molecular weight 300–800 in the rat, guinea pig, and rabbit. Biochem. J.
, 25–26 (1971).
Adamson, R. H.: Drug metabolism in marine vertebrates. Fedn Proc. Fedn Am. Socs exp. Biol.
, 1047–1055 (1967).
Barrow, A. and L. A. Griffiths: The biliary excretion of hydroxyethylrutosides and other flavonoids in the rat: Biochem. J.
, 24–25 (1971).
Bohon, R. and W. F. Clausen: The solubility of aromatic hydrocarbons in water. J. Am. chem. Soc.
, 1571–1578 (1951).
Boyland, E. and J. B. Solomon: Metabolism of polycyclic compounds. 8. Acid-labile precursors of naphthalene produced as metabolites of naphthalene. Biochem. J.
, 518–522 (1955).
Clark, H. G. and L. Diamond: Comparative studies on the interaction of benzopyrene with cells derived from poikilothermic and homeothermic vertebrates. II. Effect of temperature on benzopyrene metabolism and cell multiplication. J. Cell Physiol.
, 385–392 (1971).
Creaven, P. J., D. V. Parke and R. T. Williams: A fluorometric study of the hydroxylation of biphenyl in vitro by liver preparations of various species. Biochem. J.
, 879–885 (1965).
Diamond, L. and H. F. Clark: Comparative studies on the interaction of benzo (α) pyrone with cells derived from poikilothermic and homeothermic vertebrates. I. Metabolism of benzopyrene. J. nat. Cancer Inst.
, 1005–1011 (1970).
Falk, H. L., P. Kotin, S. S. Lee and A. Nathan: Intermediary metabolism of benzo(α)pyrene in the rat. J. natn. Cancer Inst.
, 699–745 (1962).
Gingell, R. and J. W. Bridges: Intestinal azo reduction and glucuronide conjugation of prontosil. Biochem. J.
, 24 (1971).
Gram, T. E. and J. R. Fouts: Studies on the intramicrosomal distribution of hepatic enzymes which catalyze the metabolism of drugs and other foreign compounds.
: Enzymatic oxidation of toxicants, pp 47–60. Ed. by E. Hodgson. Raleigh, North Carolina: North Carolina State University Press 1968.
Haddow, A.: Chemical carcinogens and their mode of action. Br. med. Bull.
, 79–92 (1958).
Heidelberger, C.: Studies on the molecular mechanism of hydrocarbon carcinogenesis. J. cell. comp. Physiol. (Suppl.)
, 129–148 (1964).
Jerina, D. M., J. W. Daly, B. Witkop, P. Hirenberg and S. Udenfriend: 1,2-naphthalene oxide as an intermediate in the microsomal hydroxylation of naphthalene. Biochemistry, N.Y.
, 147–156 (1970).
Johnson, R. K., W. T. Wynn and W. R. Jondorf: Some aspects of the metabolism of
C-labeled (±)-2, 3-dehydroemetine in the rat. Biochem. J.
, 26–27 (1971).
Kotin, P., H. Falk and R. Busser: Distribution, retention, and elimination of
C-3,4-benzopyrene after administration to mice and rats. J. natn. Cancer Inst.
, 541–555 (1959)
Lee, R. F., J. Hirota, J. C. Nevenzel, R. Sauerheber, A. Lewis and A. A. Benson: Lipids in the marine environment. Rep. Calif. coop. oceanic Fish. Invest.
16 (1972a). (In press).
—, R. Sauerheber and A. A. Benson: Petroleum hydrocarbons: uptake and discharge by the marine mussel,
. Science, N.Y.
, 344–346 (1972b).
Mallet, L. et M. L. Prion: Sur le retention des hydrocarbures polybenziques du type benzo-3,4-pyrene par les sediments, la faune et la flora de la Baie de Saint-Malo. C.r. hebd. Séanc. Acad. Sci., Paris
, 5264–5267 (1967).
Nelson-Smith, A.: The problem of oil pollution of the sea. Adv. mar. Biol.
, 215–306 (1970).
Ostrow, J. D.: Absorption of bile pigments by the gall bladder. J. clin. Invest.
, 2035–2052 (1967).
Remmer, H., R. W. Estabrook, J. Schenkman and H. Greim: Induction of microsomal liver enzymes:
: Enzymatic oxidation to toxicants, pp 65–85. Ed. by E. Hodgson. Raleigh, North Carolina: North Carolina State University Press 1968.
Robertson, J. S. and P. J. Dunstan: Metabolism of perhy-droanthracenes in the rabbit. Biochem. J.
, 543–547 (1971).
Shimkin, M. B., B. K. Kow and L. Zechmesiter: An instance of the occurrence of carcinogenic substances in certain barnacles. Science, N.Y.
, 650–651 (1951).
Sims, P.: The metabolism of benzopyrene by rat-liver homogenates. Biochem. Pharmac.
, 613–618 (1967).
Spencer, T. and P. W. C. Fischer: The induction of microsomal hydroxylases in regenerating rat liver. Chem. biol. Interactions.
, 41–47 (1971).
Wright, E. M. and J. M. Diamond: An electrical method for measuring non-electrolyte permeability. Proc. R. Soc. (Ser. B)
, 203–225 (1969).
Zobell, C. E.: Sources and biodegradation of carcinogenic hydrocarbons.
In. Proceedings of Joint Conference on Prevention and Control of Oil Spills, Washington, D.C., June 15–17, 1971. pp 441–451.