Cytotechnology

, Volume 3, Issue 3, pp 239–244

High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge

  • Michiyuki Tokashiki
  • Takami Arai
  • Kimihiko Hamamoto
  • Kenji Ishimaru
Original Research Papers

DOI: 10.1007/BF00365487

Cite this article as:
Tokashiki, M., Arai, T., Hamamoto, K. et al. Cytotechnology (1990) 3: 239. doi:10.1007/BF00365487

Abstract

The influence of centrifugal force on the growth of cells was examined by exposing the cells of the mouse-human hybridoma X87 line to centrifugal force (100–500 G) for ten minutes twice a day and comparing the static culture with that of unexposed cells. In this experiment, both cell proliferation and specific antibody productivity were independent of the centrifugal effect, and gave the same results as in the case of no exposure to centrifugal force. High density cultivation of the mouse-human hybridoma X87 line was obtained by a perfusion system where the cells were separated from the culture medium by continuous centrifugation. In the serum-free culture, the maximum viable cell density exceeded 107 cells/ml, and monoclonal antibody was stably produced for 37 days. The results in this culture were equivalent to those obtained by intermittent centrifugal cell separation from the culture medium, and separation by gravitational settlement.

Key words

hybridomaperfusion culturemonoclonal antibodyserum-free culturecentrifuge

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Michiyuki Tokashiki
    • 1
  • Takami Arai
    • 1
  • Kimihiko Hamamoto
    • 1
  • Kenji Ishimaru
    • 1
  1. 1.Biotechnology Research Laboratories, Tokyo Research CenterTeijin LimitedHino-shi, TokyoJapan