Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers
- Cite this article as:
- Mignot, G., Faure, T., Ganne, V. et al. Cytotechnology (1990) 4: 163. doi:10.1007/BF00365097
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Recombinant Chinese hamster ovary cells producing Von Willebrand factor have been successfully grown in gelatin macroporous microcarriers (Cultispher-G). Serum-free cultures were maintained in 1, 4, and 10 liter fermentors for more than two months. Comparative studies with Cytodex-3 microcarriers have been performed in 1 liter fermentors. The lower specific Von Willebrand factor productivity of CHO cells cultivated on Cultispher-G were offset by higher cell densities (107−2×107 cells/ml). Volumetric Von Willebrand factor productivity was influenced by oxygen concentration, and remained stable during scale-up from 1 to 10 liter fermentors.