Cytotechnology

, Volume 4, Issue 2, pp 163–171

Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers

  • G. Mignot
  • T. Faure
  • V. Ganne
  • B. Arbeille
  • A. Pavirani
  • J. L. Romet-Lemonne
Original Research Paper

DOI: 10.1007/BF00365097

Cite this article as:
Mignot, G., Faure, T., Ganne, V. et al. Cytotechnology (1990) 4: 163. doi:10.1007/BF00365097
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Abstract

Recombinant Chinese hamster ovary cells producing Von Willebrand factor have been successfully grown in gelatin macroporous microcarriers (Cultispher-G). Serum-free cultures were maintained in 1, 4, and 10 liter fermentors for more than two months. Comparative studies with Cytodex-3 microcarriers have been performed in 1 liter fermentors. The lower specific Von Willebrand factor productivity of CHO cells cultivated on Cultispher-G were offset by higher cell densities (107−2×107 cells/ml). Volumetric Von Willebrand factor productivity was influenced by oxygen concentration, and remained stable during scale-up from 1 to 10 liter fermentors.

Key words

CHO cellsmacroporous microcarrierscale-upserum-freeVon Willebrand factor

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • G. Mignot
    • 1
  • T. Faure
    • 2
  • V. Ganne
    • 1
  • B. Arbeille
    • 3
  • A. Pavirani
    • 2
  • J. L. Romet-Lemonne
    • 1
  1. 1.Centre National de Transfusion SanguineLes UlisFrance
  2. 2.StrasbourgFrance
  3. 3.U.E.R. MédecineTours, CedexFrance