Original Articles

Immunogenetics

, Volume 27, Issue 6, pp 449-455

First online:

MHC class II sequences of an HLA-DR2 narcoleptic

  • C. B. LockAffiliated withDepartment of Neurology, King's College Hospital School of Medicine, Dentistry, and Institute of Psychiatry
  • , A. K. L. SoAffiliated withHuman Immunogenetics Laboratory, Imperial Cancer Research Fund
  • , K. I. WelshAffiliated withTissue Typing, Guy's Hospital
  • , J. D. ParkesAffiliated withDepartment of Neurology, King's College Hospital School of Medicine, Dentistry, and Institute of Psychiatry
  • , J. TrowsdaleAffiliated withHuman Immunogenetics Laboratory, Imperial Cancer Research Fund

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Abstract

Narcolepsy has a 98% association with the DR2-Dw2/DQw1 haplotype. To establish if a disease-specific allele is present in narcolepsy, a cDNA library was made from a B-cell line from a DR2,4/DQw1,3 narcoleptic. Clones encoding the two expressed DR2 β chains, along with DQw1 α and β chains, were isolated and completely sequenced. The coding regions of these four genes were similar to published nucleotide and protein sequences from corresponding healthy controls, with some minor exceptions. The 3′ untranslated region of one of the DR2 β genes in the narcoleptic was extended by 42 bp. Complete sequences were not available for DQw1.2 α or β from healthy individuals, but first domain nucleotide sequences showed only a single nonproductive difference in DQα. Partial protein sequences of both DQ α and β from published data were identical. Although the effects of minor differences cannot be ruled out completely, it is concluded that there are probably no narcolepsy-specific DR β or DQ α/β sequences, and that the alleles found in narcolepsy are representative of those found in the healthy population.