Genetic organization of the ovine MHC class II region
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- Scott, P.C., Choi, CL. & Brandon, M.R. Immunogenetics (1987) 25: 116. doi:10.1007/BF00364277
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To study the class II genes of the major histocompatibility region of the sheep genome, human HLA class II genes corresponding to the known subregions in man (DR, DQ, DP, DO, and DZ) were used for Southern hybridization analysis of sheep DNA and to probe a sheep genomic library. Hybridizing bands were noted for all probes except DPα. DQ α and β and DRβ appear to be present as multicopy genes, while DRα-, DZα-, , and DOβ-like genes appear to be single copy. All bands detected with the DPβ probe were also detectable with other β chain probes. From eight λ-bacteriophage clones of a sheep genomic library nine distinct class II genes were identified. These genes were characterized by differential hybridization analysis and restriction mapping. Two genes were DRβ-like, three DQα-like and four DQβ-like. The extensive cross-hybridization observed with β chain probes was not seen with α chain probes. The results of this study suggest that the major histocompatibility complex class II region of the sheep has a similar genetic organization to that of man, with the provisional exception of the DP subregion.
Abbreviation used in this paper
ovine major histocompatibility complex